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miRNA-101 Targets TGF-βR1 to Retard the Progression of Oral Squamous Cell Carcinoma by Yong Wang; Rui-Zhi Jia; Shu Diao; Jun He; Li Jia is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is miRNA-101 Targets TGF-βR1 to Retard the Progression of Oral Squamous Cell Carcinoma about?

Despite the considerable knowledge on the involvement of microRNA-101 (miR-101) in the evolution of oral squamous cell carcinoma (OSCC), the underlying mechanisms remain obscure. In this study, miR-101 expression was markedly downregulated in the OSCC cell lines and tissues. Cell counting kit-8 (CCK-8), ethynyl deoxyuridine (EdU), and colony formation assays showed that miR-101 inhibited the proliferation of OSCC cells. Flow cytometry and caspase 3 activity assays indicated that miR-101 induced OSCC cell apoptosis. Transwell assays demonstrated that this miRNA also repressed OSCC cell migration and invasion. Moreover, tube formation assay showed that miR-101 abated the proangiogenesis of OSCC cells. Dual-luciferase reporter assay confirmed that miR-101 directly targeted transforming growth factor-β receptor 1 (TGF-βR1) in OSCC. Ectopic expression of TGF-βR1 counteracted the effects of miR-101 on the OSCC cell characteristics. Thus, miR-101 significantly abolished the proliferation, motility, and proangiogenesis of OSCC cells and induced their apoptosis by targeting TGF-βR1. These results imply the potential application of miR-101 in OSCC treatment.

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Author

Yong Wang; Rui-Zhi Jia; Shu Diao; Jun He; Li Jia

Publisher

Computers, Materials and Continua (Tech Science Press)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)