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Can I read An N-terminally fused Xenopus transcription factor IIIA synthesized in Escherichia coli is biologically active. on EtoBox?
An N-terminally fused Xenopus transcription factor IIIA synthesized in Escherichia coli is biologically active. by C I Pao; T C Lee; Y D Liao; C W Wu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is An N-terminally fused Xenopus transcription factor IIIA synthesized in Escherichia coli is biologically active. about?
A 1.5-kilobase DNA fragment containing the Xenopus transcription factor IIIA (TFIIIA) gene was inserted into the prokaryotic expression vector pIN-III(A) containing the lpp/lac promoter. The recombinant DNA was introduced into Escherichia coli K-12 strain SB221. The expression TFIIIA gene was induced by isopropyl-beta-D-thiogalactopyranoside, which resulted in the synthesis of a recombinant TFIIIA with an extra 17 amino acids fused to its N terminus as predicted from the nucleotide sequence. The engineered gene product, purified to at least 90% homogeneity, retained its binding affinity to the intragenic control region of the 5 S RNA gene, as well as its activity to stimulate 5 S RNA gene transcription in vitro.
Who reads An N-terminally fused Xenopus transcription factor IIIA synthesized in Escherichia coli is biologically active.?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- C I Pao; T C Lee; Y D Liao; C W Wu
- Publisher
- Elsevier BV
- Published
- 1988
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)