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Open neural tube defects: Immunocytochemical demonstration of neuroepithelial cells in amniotic fluid by Ellen Greenebaum; Mahesh M. Mansukhani; Debra S. Heller; Ilan Timor-Tristsch is a Medicine article available to read on EtoBox.

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## Cytologic evaluation of second trimester amniotic fluid (AF) is a rapid, inexpensive adjunct to prenatal diagnosis of open neural tube defects (ONTDs). Our goal was to determine whether the neural-appearing cells and/or large foamy macrophages in the AF of anencephalics are indeed of neural and/or glial origin. In two second trimester patients with elevated serum alpha-fetoprotein (AFP) and polyhydramnios, fetal sonogram studies showed anencephaly; amniocentesis was performed for AF-AFP, cytogenetic, and cytologic studies. AF sediment smears were initially Papanicolaou-stained; next, the same smears were immunoperoxidase (IP)-stained for glial fibrillary acidic protein (GFAP). If GFAP negative, slides were restained for synaptophysin (SYN) and neuron-specific enolase (NSE). Both AFs contained small neuralappearing cells (5-10 mm) singly and in clusters, with dense, round, homogeneous nuclei, an occasional nucleolus, and scant cytoplasmic rim. These were GFAP negative and SYN and NSE positive; the large vacuolated, lipid-laden macrophages (20-40 mm) were negative for all three IP stains. In conclusion, positive IP staining for SYN and NSE supports the morphologic impression that

Who reads Open neural tube defects: Immunocytochemical demonstration of neuroepithelial cells in amniotic fluid?

Author

Ellen Greenebaum; Mahesh M. Mansukhani; Debra S. Heller; Ilan Timor-Tristsch

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 8755-1039)

Published

1997

Language

EN

Field

Medicine (Health Sciences)