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Regioselective hydroxylation of daidzein using P450 (CYP105D7) from Streptomyces avermitilis MA4680 by Bishnu Prasad Pandey; Changhyun Roh; Kwon-Young Choi; Nahum Lee; Eun Jung Kim; Sungghi Ko; Taejin Kim; Hyundon Yun; Byung-Gee Kim is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Regioselective hydroxylation of daidzein using P450 (CYP105D7) from Streptomyces avermitilis MA4680 about?
## Abstract Regiospecific 3′‐hydroxylation reaction of daidzein was performed with CYP105D7 from __Streptomyces avermitilis__ MA4680 expressed in __Escherichia coli__. The apparent __K__~m~ and __k__~cat~ values of CYP105D7 for daidzein were 21.83 ± 6.3 μM and 15.01 ± 0.6 min^−1^ in the presence of 1 μM of CYP105D7, putidaredoxin (CamB) and putidaredoxin reductase (CamA), respectively. When CYP105D7 was expressed in __S. avermitilis__ MA4680, its cytochrome P450 activity was confirmed by the CO‐difference spectra at 450 nm using the whole cell extract. When the whole‐cell reaction for the 3′‐hydroxylation reaction of daidzein was carried out with 100 μM of daidzein in 100 mM of phosphate buffer (pH 7.5), the recombinant __S. avermitilis__ grown in R2YE media overexpressing CYP105D7 and ferredoxin FdxH (SAV7470) showed a 3.6‐fold higher conversion yield (24%) than the corresponding wild type cell (6.7%). In a 7 L (working volume 3 L) jar fermentor, the recombinants __S. avermitilis__ grown in R2YE media produced 112.5 mg of 7,3′,4′‐trihydroxyisoflavone (i.e., 29.5% conversion yield) from 381 mg of daidzein in 15 h. Biotechnol. Bioeng. 2010. 105: 697–704. © 2009 Wiley Periodicals.
Who reads Regioselective hydroxylation of daidzein using P450 (CYP105D7) from Streptomyces avermitilis MA4680?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Bishnu Prasad Pandey; Changhyun Roh; Kwon-Young Choi; Nahum Lee; Eun Jung Kim; Sungghi Ko; Taejin Kim; Hyundon Yun; Byung-Gee Kim
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)