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Real-Time monitoring of intracellular wax ester metabolism by Suvi Santala; Elena Efimova; Matti Karp; Ville Santala is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Real-Time monitoring of intracellular wax ester metabolism about?

## Background Wax esters are industrially relevant molecules exploited in several applications of oleochemistry and food industry. At the moment, the production processes mostly rely on chemical synthesis from rather expensive starting materials, and therefore solutions are sought from biotechnology. Bacterial wax esters are attractive alternatives, and especially the wax ester metabolism of __Acinetobacter__ sp. has been extensively studied. However, the lack of suitable tools for rapid and simple monitoring of wax ester metabolism __in vivo__ has partly restricted the screening and analyses of potential hosts and optimal conditions. ## Results Based on sensitive and specific detection of intracellular long-chain aldehydes, specific intermediates of wax ester synthesis, bacterial luciferase (LuxAB) was exploited in studying the wax ester metabolism in __Acinetobacter baylyi__ ADP1. Luminescence was detected in the cultivation of the strain producing wax esters, and the changes in signal levels could be linked to corresponding cell growth and wax ester synthesis phases. ## Conclusions The monitoring system showed correlation between wax ester synthesis pattern and luminescent signa

Who reads Real-Time monitoring of intracellular wax ester metabolism?

Author

Suvi Santala; Elena Efimova; Matti Karp; Ville Santala

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2002-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1475-2859)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)