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Regulation of Fibronectin and Laminin Receptor Expression, Fibronectin and Laminin Secretion in Human Colon Cancer Cells by Transforming Growth Factor‐β~1~ by Shuang Huang; Subhas Chakrabarty is a Medicine article available to read on EtoBox.

What is Regulation of Fibronectin and Laminin Receptor Expression, Fibronectin and Laminin Secretion in Human Colon Cancer Cells by Transforming Growth Factor‐β~1~ about?

## Abstract Transforming growth factor (TGF)‐β~1~, modulates the expression of extracellular matrix (ECM) glycoproteins, fibronectin and laminin and the adhesion of Moser colon cancer cells to these glycoproteins. Since adhesion can be altered through expression of cell‐surface receptors, binding affinities of adhesion molecules for receptors, or both, we investigated the effect of TGF‐β~1~, on the binding properties of fibronectin and laminin to their cell‐surface receptors by saturation binding and Scatchard analyses using radiolabeled fibronectin and laminin. Fibronectin bound to its cell‐surface receptor with high affinity (K~d~ = 1.25 × 10^−9^ M), Moser cells had approximately 7.1 × 10^4^ fibronectin‐binding sites per cell. TGF‐β~1~ treatment rapidly up‐modulated the number of cell‐surface fibronectin‐binding sites by 1.9‐fold. The binding affinity of fibronectin for the receptor, however, was not altered. Laminin was found to bind to a higher‐affinity and a lower‐affinity receptor. Moser cells expressed approximately 1.1 × 10^3^ higher‐affinity laminin‐binding sites and approximately 3.1 × 10 lower‐affinity‐binding sites per cell. TGF‐β~1~ rapidly increased the expression of

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Author

Shuang Huang; Subhas Chakrabarty

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1994

Language

EN

Field

Medicine (Health Sciences)