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Oligomeric interaction of the PapB transcriptional regulator with the upstream activating region of pili adhesin gene promoters in Escherichia coli by Yan Xia; Kristina Forsman; Jana Jass; Bernt Eric Uhlin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Oligomeric interaction of the PapB transcriptional regulator with the upstream activating region of pili adhesin gene promoters in Escherichia coli about?
Transcriptional regulation of the __pap__ genes, which encode fimbrial adhesins in uropathogenic __Escherichia coli__, depends on an upstream activating region. This region contains binding sites for a transcription factor, PapB, which is a member of a growing family of putative regulatory proteins found in several virulence‐associated fimbrial gene systems. To assess the nature of the PapB binding sites, we studied different naturally occurring variants and a number of __in vitro__ constructed mutant binding sites. DNase I footprinting analysis and visualization of the PapB–DNA complex by atomic force microscopy showed that the protein occupied a DNA region of more than 50 bp. Purified PapB protein was shown to recognize a motif including a 9 bp repeat sequence containing T/A triplets at a conserved position. PapB binding was affected by distamycin, and the results were consistent with the possibility that the binding to DNA occurred through minor groove interaction. From these analyses and estimation of the relative number of PapB proteins per binding site, we suggest that PapB binds the DNA in an oligomeric fashion and may function as an architectural factor in the transcription
Who reads Oligomeric interaction of the PapB transcriptional regulator with the upstream activating region of pili adhesin gene promoters in Escherichia coli?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Yan Xia; Kristina Forsman; Jana Jass; Bernt Eric Uhlin
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)
- Published
- 1998
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)