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Hsp101 Is Necessary for Heat Tolerance but Dispensable for Development and Germination in the Absence of Stress by Suk‐Whan Hong; Elizabeth Vierling is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary Hsp101 is a molecular chaperone that is required for the development of thermotolerance in plants and other organisms. We report that __Arabidopsis thaliana__ Hsp101 is also regulated during seed development in the absence of stress, in a pattern similar to that seen for LEA proteins and small Hsps; protein accumulates during mid‐maturation and is stored in the dry seed. Two new alleles of the locus encoding Hsp101 (__HOT1__) were isolated from __Arabidopsis__ T‐DNA mutant populations. One allele, __hot1‐3__, contains an insertion within the second exon and is null for Hsp101 protein expression. Despite the complete absence of Hsp101 protein, plant growth and development, as well as seed germination, are normal, demonstrating that Hsp101 chaperone activity is not essential in the absence of stress. In thermotolerance assays __hot1‐3__ shows a similar, though somewhat more severe, phenotype to the previously described missense allele __hot1‐1__, revealing that the __hot1‐1__ mutation is also close to null for protein activity. The second new mutant allele, __hot1‐2,__ has an insertion in the promoter 101 bp 5′ to the putative TATA element. During heat stress the __hot1‐2_

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Author

Suk‐Whan Hong; Elizabeth Vierling

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

2001

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)