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Expression and characterization of recombinant vitamin K-dependent γ-glutamyl carboxylase from an invertebrate, Conus textile by Eva Czerwiec; Gail S. Begley; Mila Bronstein; Johan Stenflo; Kevin Taylor; Barbara C. Furie; Bruce Furie is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Expression and characterization of recombinant vitamin K-dependent γ-glutamyl carboxylase from an invertebrate, Conus textile about?
The marine snail __Conus__ is the sole invertebrate wherein both the vitamin K‐dependent carboxylase and its product, γ‐carboxyglutamic acid, have been identified. To examine its biosynthesis of γ‐carboxyglutamic acid, we studied the carboxylase from __Conus__ venom ducts. The carboxylase cDNA from __Conus textile__ has an ORF that encodes a 811‐amino‐acid protein which exhibits sequence similarity to the vertebrate carboxylases, with 41% identity and ≈ 60% sequence similarity to the bovine carboxylase. Expression of this cDNA in COS cells or insect cells yielded vitamin K‐dependent carboxylase activity and vitamin K‐dependent epoxidase activity. The recombinant carboxylase has a molecular mass of ≈ 130 kDa. The recombinant __Conus__ carboxylase carboxylated Phe‐Leu‐Glu‐Glu‐Leu and the 28‐residue peptides based on residues −18 to +10 of human proprothrombin and proFactor IX with __K__~m~ values of 420 μm, 1.7 μm and 6 μm, respectively; the __K__~m~ for vitamin K is 52 μm. The __K__~m~ values for peptides based on the sequence of the conotoxin ε‐TxIX and two precursor analogs containing 12 or 29 amino acids of the propeptide region are 565 μm, 75 μm and 74 μm, respectively. The reco
Who reads Expression and characterization of recombinant vitamin K-dependent γ-glutamyl carboxylase from an invertebrate, Conus textile?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Eva Czerwiec; Gail S. Begley; Mila Bronstein; Johan Stenflo; Kevin Taylor; Barbara C. Furie; Bruce Furie
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)