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Characterization of an extracellular alkaline lipase from Pseudomonas mendocina M‐37 by Praveen Dahiya; Pooja Arora; Ashok Chaudhury; Subhash Chand; Neeraj Dilbaghi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract A strain of __Pseudomonas mendocina__ producing extracellular lipase was isolated from soil. The bacterium accumulates lipase in culture fluid when grown aerobically at 30 °C for 24 h in a medium composed of olive oil (1%) as substrate. __Pseudomonas mendocina__ lipase was optimally active at pH 9.0, temperature of 50 °C and was found to be stable between pH 7.0–11.0. The lipase was inhibited by detergents such as SDS and Tween‐80. The enzyme was stable in various organic solvents tested with maximum stability in chloroform followed by toluene and exhibited 1–3 regiospecificity for hydrolytic reaction. This lipase was capable of hydrolyzing a variety of lipidic substrates and is mainly active towards synthetic triglycerides and fatty acid esters that possess a butyryl group. Metal ions like Mg^2+^, Ca^2+^ and Na^+^ stimulated lipase activity, whereas, Cu^2+^, Mn^2+^ and Hg^2+^ ions caused inhibition. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

Who reads Characterization of an extracellular alkaline lipase from Pseudomonas mendocina M‐37?

Author

Praveen Dahiya; Pooja Arora; Ashok Chaudhury; Subhash Chand; Neeraj Dilbaghi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Akademie Verlag; Wiley (ISSN 0233-111X)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)