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Enhanced Inflammatory Responses to Toll-like Receptor 2/4 Stimulation in Type 1 Diabetic Coronary Artery Endothelial Cells: the Effect of Insulin by Jilin Li; Chunhua Jin; Joseph C Cleveland; Lihua Ao; Dingli Xu; David A Fullerton; Xianzhong Meng is a Medicine article available to read on EtoBox.
What is Enhanced Inflammatory Responses to Toll-like Receptor 2/4 Stimulation in Type 1 Diabetic Coronary Artery Endothelial Cells: the Effect of Insulin about?
## Background Endothelial inflammatory responses mediated by Toll-like receptors (TLRs), particularly TLR2 and TLR4, play an important role in atherogenesis. While Type 1 diabetes (T1D) promotes the development and progression of atherosclerosis, the effect of T1D on TLR2/4-mediated inflammatory responses in coronary artery endothelial cells (CAECs) remains unclear. ## Methods We tested the hypothesis that diabetic CAECs have enhanced inflammatory responses to TLR2/4 stimulation. Non-diabetic and diabetic CAECs were treated with TLR2 agonist peptidoglycan and TLR4 agonist lipopolysaccharide. The expression of ICAM-1, IL-6 and IL-8 were analyzed by real-time PCR, immunoblotting and ELISA, and NF-κB activation by immunoblotting and immunostaining. In additional experiments, insulin was added before TLR stimulation to determine whether insulin deficiency alone is responsible for the alteration of TLR2/4-mediated inflammatory responses. ## Results Stimulation of TLR2 or TLR4 induced NF-κB activation, and the expression of ICAM-1, IL-6 and IL-8. Interestingly, the expression of inflammatory mediators was significantly enhanced in diabetic cells. The enhanced inflammatory responses corre
Who reads Enhanced Inflammatory Responses to Toll-like Receptor 2/4 Stimulation in Type 1 Diabetic Coronary Artery Endothelial Cells: the Effect of Insulin?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Jilin Li; Chunhua Jin; Joseph C Cleveland; Lihua Ao; Dingli Xu; David A Fullerton; Xianzhong Meng
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2002-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1475-2840)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)