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Urease immobilization on magnetic micro/nano-cellulose dialdehydes: Urease inhibitory of Biginelli product in Hantzsch reaction by urea by Fatemeh Tamaddon; Davood Arab; Ehsan Ahmadi-AhmadAbadi is a Materials Science article available to read on EtoBox.

What is Urease immobilization on magnetic micro/nano-cellulose dialdehydes: Urease inhibitory of Biginelli product in Hantzsch reaction by urea about?

Micro/nano celluloses (MC)/NC) were magnetized by nanoγ-FeO into the nanoγ-FeO@MC (NMMC) and nanoγ-FeO@NC (NMMC) which oxidized to NMMCD and NMNCD dialdehydes for Schiff-base immobilization of urease as NMMCD/urease and NMMCD/urease. The relative enzyme-activity of the immobilized ureases were comparable with the free-urease, although 75%-80% of the enzyme activity preserved for NMMCD/urease and NMNCD/urease after six cycles. The compared catalytic activities of the NMMCD/urease and NMMCD/urease in Biginelli/Hantzsch reactions in water at 60 °C surprised us by 100% selectivity for the Biginelli product 3,4-dihydropyrimidin-2(1H)-one (DHPM). With the superiority of NMNCD/urease, this high selectivity using immobilized ureases is owing to the admirable urease inhibitory of the formed Biginelli product DHPM by urea condensation instead of urea hydrolysis. The robust enzyme inhibitory of the DHPM for free urease was also confirmed by phenol red test to show the deactivation of enzyme for enzymatic hydrolysis of urea and ammonia production in water.

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Author

Fatemeh Tamaddon; Davood Arab; Ehsan Ahmadi-AhmadAbadi

Publisher

Elsevier BV

Published

2019

Language

EN

Field

Materials Science (Physical Sciences)