About this Medicine article
Dopamine induces ERK activation in renal epithelial cells through H2O2 produced by monoamine oxidase by Vindis, Cécile; Séguélas, Marie-Hélène; Lanier, Stephen; Parini, Angelo; Cambon, Claudie is a Medicine article available to read on EtoBox.
## Background: The rat renal proximal tubule cells contain a large amount of monoamine oxidase, which catalyzes the oxidative deamination of catecholamines such as dopamine (da). the aim of this study is to investigate the potential role of hydrogen peroxide (h2o2) produced by monoamine oxidase (mao) isoform on regulation of cell signaling and function. ## Methods: Primary rat proximal tubular cells, which contain almost exclusively mao-a, and human embryonic kidney 293 (hek 293) cells stably transfected with human mao-b cdna were treated with da or tyramine in the presence or the absence of some inhibitors. then, shc protein tyrosine phosphorylation and extracellular-regulated kinase (erk) activation were evaluated by immunoprecipitation/immunoblot analysis and cell proliferation by [3h]thymidine incorporation or cell counting. ## Results: In rat proximal tubule cells, da induced tyrosine phosphorylation of shc, erk activation, and a significant increase in dna synthesis. the involvement of mao-dependent h2o2 generation induced by da (5 micromol/l) was supported by the demonstration that the da effects were (1) fully prevented by cell pretreatment with the mao inhibitor pargyline,
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- Author
- Vindis, Cécile; Séguélas, Marie-Hélène; Lanier, Stephen; Parini, Angelo; Cambon, Claudie
- Publisher
- Nature Publishing Group; Elsevier ; Elsevier Inc.; Elsevier BV; ScienceOpen (ISSN 0085-2538)
- Published
- 2001
- Language
- EN
- Field
- Medicine (Health Sciences)