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Simultaneous determination of saponins in flower buds of Panax notoginseng using high performance liquid chromatography by Xianfu Gao; Mo Dan; Aihua Zhao; Guoxiang Xie; Wei Jia is a Chemistry article available to read on EtoBox.

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The flower buds of Panax notoginseng have been commonly used for the treatment of hypertension, vertigo, tinnitus and acute faucitis in China. The amount of total saponins in the flower buds is higher than in any other parts of P. notoginseng. However, the compositions of flower buds have not been quantified clearly until now. A sensitive and efficient high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed for the first time to simultaneously quantify eight active saponins in the flower buds of P. notoginseng, including notoginsenoside R(1) and ginsenosides Rg(1), Re, Rb(1), Rb(2), Rb(3), Rd and F(2). The analysis was performed on a reversed-phase C(18) column with gradient elution of acetonitrile and 0.01% aqueous formic acid. The proposed method provided good linearity, reproducibility and sensitivity for the simultaneous quantification of the investigated saponins with overall intra- and inter-day precision and accuracy of better than 4.1% (RSD) and higher than 95% (accuracy), respectively. The recoveries for all the saponins determined were in the range 94.7-104.8% with RSD better than 3.1%. Using the optimized method, we were able to analyze samples

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Author

Xianfu Gao; Mo Dan; Aihua Zhao; Guoxiang Xie; Wei Jia

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0269-3879)

Published

2007

Language

EN

Field

Chemistry (Physical Sciences)