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Adult parasite burden and excretion of first-stage larvae of Angiostrongylus vasorum in dogs: Methodologically relevant diagnostic aspects and associations with serological detection of parasite antigen and specific antibodies by Andreas W. Oehm; Manuela Schnyder is a Immunology and Microbiology article available to read on EtoBox.
What is Adult parasite burden and excretion of first-stage larvae of Angiostrongylus vasorum in dogs: Methodologically relevant diagnostic aspects and associations with serological detection of parasite antigen and specific antibodies about?
Angiostrongylus vasorum is a widely distributed cardiopulmonary parasite of canids in Europe. Clinical signs in dogs can be highly variable and diagnostically challenging. A correct and early diagnosis is hence indispensable to adequately manage affected patients. First-stage larvae (L1) are excreted in the faeces of definitive hosts and conventionally identified using the Baermann technique. Moreover, ELISAs for the detection of circulating antigen and specific antibodies have been presented. The current study aimed at i) quantitatively assessing larval migration in the Baermann funnel after 12 h and 24 h; ii) investigating the influence of sample storage at 4 °C over the course of three days on the number of detected L1; iii) evaluating potential associations of adult worm burdens with larval shedding in dogs and ELISA optical density (OD) values for circulating parasite antigen and specific antibodies. Faecal samples were obtained from naturally infected dogs (n = 21) and Baermann funnels were set up in duplicate over the course of four consecutive days (days 0-3) starting with the day of sample collection. Funnels were harvested on days 1-4 after 12 and 24 h, respectively, and
Who reads Adult parasite burden and excretion of first-stage larvae of Angiostrongylus vasorum in dogs: Methodologically relevant diagnostic aspects and associations with serological detection of parasite antigen and specific antibodies?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Andreas W. Oehm; Manuela Schnyder
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)