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Cytotoxicity Testing of Materials with Limited in Vivo Exposure Is Affected by the Duration of Cell–material Contact by Gabriela Ciapetti; Donatella Granchi; Susanna Stea; Lucia Savarino; Elisabetta Verri; Alessandra Gori; Franca Savioli; Lucio Montanaro is a Medicine article available to read on EtoBox.

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Silicones for dental impression largely are used to record the geometry of hard and soft dental tissues. They are considered to be medical devices, and the assessment of cytotoxicity is a necessary step in the evaluation of their biocompatibility. Extracts of six addition-type and six condensation-type silicones have been tested with L929 cells according to the ISO 10993-Part 5 standard. The cytotoxicity was evaluated by three different methods: neutral red uptake, propidium iodide (PI) staining, and amido black staining. According to the selected specific assay, contact between cells and material extracts was maintained for 24 h in the first series of experiments; then, considering that in vivo application of these materials is restricted to a few minutes, additional experiments were performed after 1 h of cell/extract contact. Analysis of the results showed that the addition-type silicones are nontoxic even when tested after prolonged exposure of the cells to the materials while the condensation-type silicones were cytotoxic at 24 h of incubation. Nevertheless, harm to the patient actually could be negligible, considering its very short time of exposure in vivo. This is supported

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Author

Gabriela Ciapetti; Donatella Granchi; Susanna Stea; Lucia Savarino; Elisabetta Verri; Alessandra Gori; Franca Savioli; Lucio Montanaro

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Heterocorporation; Wiley (ISSN 0021-9304)

Published

1998

Language

EN

Field

Medicine (Health Sciences)