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When Two Into One Won't Go: Fitting in the Presence of Steric Hindrance and Partial Occupancy by Eric Thouvenin; Elizabeth Hewat is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Combining structural data from cryo-electron microscopy (cryo-EM) and X-ray crystallography to give pseudo-atomic models of large molecular complexes has proved particularly suitable for studying viruses and viral complexes. Several groups are developing programs to fit X-ray data to EM data. These programs are in general tailored to particular problems with regard to size, symmetry, number of rigid bodies, resolution __etc__. Here, two approaches are described to fitting X-ray data to EM data in the presence of steric interference and their relative merits and limitations are indicated. These fitting techniques are applied to the case of the rotavirus double-layered particle (DLP) in complex with antibodies which inhibit the transcription of mRNA by the DLP. This is a particularly good test case, as the cryo-electron microscopy map of the DLP–Fab complex, the X-ray structure of the viral protein (VP6) and also that of the VP6–Fab complex are available. The estimation of partial occupancy is also considered.

Who reads When Two Into One Won't Go: Fitting in the Presence of Steric Hindrance and Partial Occupancy?

Author

Eric Thouvenin; Elizabeth Hewat

Publisher

International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)