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The Influence of Diffusion in the External Medium Upon the Determination of Cytoplasmic Diffusion Coefficients by Søren Løvtrup; Kjell Hansson Mild; André Berglund is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The exchange of isotopic water has been studied in ovarian eggs from three Anuran species (__R. esculenta, R. pipiens__ and __R. temporaria__) by the automatic diver balance technique. The oocytes were treated with chemicals (digitonin, ethanol and formaldehyde) to remove the plasma membrane. The cytoplasmic diffusion coefficient for water was calculated with and without allowance for the influence of diffusion in the external medium. The former expedient was found necessary in order to obtain correct values. The chemical treatments gave different values, but there are sound reasons to believe that those obtained with digitonin (8.9, 15.3 and 10.8.10^−6^ cm^2^sec^−1^ for __R. esculenta, R. pipiens__ and __R. temporaria__, respectively) are the correct ones. These results indicate that the rate of diffusion in the oocyte cytoplasm amounts to 50–75% of that of free diffusion.

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Author

Søren Løvtrup; Kjell Hansson Mild; André Berglund

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1970

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)