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Viral serotype and the transgene sequence influence overlapping adeno‐associated viral (AAV) vector‐mediated gene transfer in skeletal muscle by Arkasubhra Ghosh; Yongping Yue; Dongsheng Duan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Viral serotype and the transgene sequence influence overlapping adeno‐associated viral (AAV) vector‐mediated gene transfer in skeletal muscle about?

## Abstract ## Background The overlapping approach was developed recently to expand the adeno‐associated viral (AAV) packaging capacity. In this approach, a gene is split into two partially overlapping fragments and separately packaged into an upstream and a downstream vector, respectively. Transgene expression is achieved in co‐infected cells after homologous recombination. Despite the promising proof‐of‐principle results in the lung, the efficiency has been very disappointing in skeletal muscle. Here we examined two potential rate‐limiting factors including AAV serotype and the transgene sequence. ## Methods To study serotype effect, we delivered AAV‐2, ‐5 and ‐6 overlapping vectors (5 × 10^8^ vg particles of the upstream and the downstream vectors, respectively) and 5 × 10^8^ vg particles of the intact gene vector to the tibialis anterior muscles of 7‐week‐old C57Bl/6 mice, respectively. To determine the effect of transgene sequence, we compared LacZ and alkaline phosphatase (AP) overlapping vectors. Transduction efficiency was quantified 6 weeks later by scoring the percentage of transgene‐positive myofibers. ## Results AAV‐2 overlapping vectors barely resulted in detectable tr

Who reads Viral serotype and the transgene sequence influence overlapping adeno‐associated viral (AAV) vector‐mediated gene transfer in skeletal muscle?

Author

Arkasubhra Ghosh; Yongping Yue; Dongsheng Duan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)