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HIV DNA Sequencing to Detect Archived Antiretroviral Drug Resistance by Anna Maria Geretti; Jose Luis Blanco; Anne Genevieve Marcelin; Carlo Federico Perno; Hans Jurgen Stellbrink; Dan Turner; Tuba Zengin is a Medicine article available to read on EtoBox.

What is HIV DNA Sequencing to Detect Archived Antiretroviral Drug Resistance about?

## Introduction Proviral HIV DNA integrated within CD4 T-cells maintains an archive of viral variants that replicate during the course of the infection, including variants with reduced drug susceptibility. We considered studies that investigated archived drug resistance, with a focus on virologically suppressed patients and highlighted interpretative caveats and gaps in knowledge. ## Results Either Sanger or deep sequencing can be used to investigate resistance-associated mutations (RAMs) in HIV DNA recovered from peripheral blood. Neither technique is free of limitations. Furthermore, evidence regarding the establishment, maintenance, expression and clinical significance of archived drug-resistant variants is conflicting. This in part reflects the complexity of the HIV proviral landscape and its dynamics during therapy. Clinically, detection of RAMs in cellular HIV DNA has a variable impact on treatment outcomes, modulated by the drugs affected, treatment duration and additional determinants of virological failure, including those leading to suboptimal drug exposure. ## Conclusions Sequencing cellular HIV DNA can provide helpful complementary information in treatment-experienced p

Who reads HIV DNA Sequencing to Detect Archived Antiretroviral Drug Resistance?

Author

Anna Maria Geretti; Jose Luis Blanco; Anne Genevieve Marcelin; Carlo Federico Perno; Hans Jurgen Stellbrink; Dan Turner; Tuba Zengin

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Medicine (Health Sciences)