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Growth Inhibition of Mammalian Cells by Eosinophil Cationic Protein by Takashi Maeda; Midori Kitazoe; Hiroko Tada; Rafael de Llorens; David S. Salomon; Masakazu Ueda; Hidenori Yamada; Masaharu Seno is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Growth Inhibition of Mammalian Cells by Eosinophil Cationic Protein about?
Eosinophil cationic protein (ECP), one of the major components of basic granules of eosinophils, is cytotoxic to tracheal epithelium. However, the extent of this effect on other cell types has not been evaluated __in vitro__. In this study, we evaluated the effect of ECP on 13 mammalian cell lines. ECP inhibited the growth of several cell lines including those derived from carcinoma and leukemia in a dose‐dependent manner. The IC~50~ values on A431 cells, MDA‐MB‐453 cells, HL‐60 cells and K562 cells were␣estimated to be ≈ 1–5 μm. ECP significantly suppressed the size of colonies of A431 cells, and decreased K562 cells in G~1~/G~0~ phase. However, there was little evidence that ECP killed cells in either cell line. These effects of ECP were not enhanced by extending its N‐terminus. Rhodamine B isothiocyanate‐labeled ECP started to bind to A431 cells after 0.5 h and accumulated for up to 24 h, indicating that specific affinity for the cell surface may be important. The affinity of ECP for heparin was assessed and found to be reduced when tryptophan residues, one of which is located at a position in the catalytic subsite of ribonuclease in ECP, were modified. The growth‐inhibitory eff
Who reads Growth Inhibition of Mammalian Cells by Eosinophil Cationic Protein?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Takashi Maeda; Midori Kitazoe; Hiroko Tada; Rafael de Llorens; David S. Salomon; Masakazu Ueda; Hidenori Yamada; Masaharu Seno
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)