Opening book details…
Can I read Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis on EtoBox?
Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis by Syed Muhammad Muntazir Mehdi; Sivakumar Krishnamoorthy; Michal Wojciech Szczesniak; Agnieszka Ludwików is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis about?
miRNAs are involved in various biological processes, including adaptive responses to abiotic stress. To understand the role of miRNAs in the response to ABA, ABA-responsive miRNAs were identified by small RNA sequencing in wild-type __Arabidopsis__, as well as in __abi1td__, __mkkk17__, and __mkkk18__ mutants. We identified 10 novel miRNAs in WT after ABA treatment, while in __abi1td__, __mkkk17__, and __mkkk18__ mutants, three, seven, and nine known miRNAs, respectively, were differentially expressed after ABA treatment. One novel miRNA (miRn-8) was differentially expressed in the __mkkk17__ mutant. Potential target genes of the miRNA panel were identified using psRNATarget. Sequencing results were validated by quantitative RT-PCR of several known and novel miRNAs in all genotypes. Of the predicted targets of novel miRNAs, seven target genes of six novel miRNAs were further validated by 5′ RLM-RACE. Gene ontology analyses showed the potential target genes of ABA-responsive known and novel miRNAs to be involved in diverse cellular processes in plants, including development and stomatal movement. These outcomes suggest that a number of the identified miRNAs have crucial roles in pla
Who reads Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Syed Muhammad Muntazir Mehdi; Sivakumar Krishnamoorthy; Michal Wojciech Szczesniak; Agnieszka Ludwików
- Publisher
- MDPI AG
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)