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Efficient production of retinol in Yarrowia lipolytica by increasing stability using antioxidant and detergent extraction by Hyemin Park; Dongpil Lee; Jae-Eung Kim; Seonmi Park; Joo Hyun Park; Cheol Woong Ha; Minji Baek; Seok-Hwan Yoon; Kwang Hyun Park; Peter Lee; Ji-Sook Hahn is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Efficient production of retinol in Yarrowia lipolytica by increasing stability using antioxidant and detergent extraction about?
The demand for bio-based retinol (vitamin A) is currently increasing, however its instability represents a major bottleneck in microbial production. Here, we developed an efficient method to selectively produce retinol in Yarrowia lipolytica. The β-carotene 15,15'-dioxygenase (BCO) cleaves β-carotene into retinal, which is reduced to retinol by retinol dehydrogenase (RDH). Therefore, to produce retinol, we first generated β-carotene-producing strain based on a high-lipid-producer via overexpressing genes including heterologous β-carotene biosynthetic genes, GGS1 mutant of endogenous geranylgeranyl pyrophosphate synthase isolated by directed evolution, and FAD1 encoding flavin adenine dinucleotide synthetase, while deleting several genes previously known to be beneficial for carotenoid production. To produce retinol, 11 copies of BCO gene from marine bacterium 66A03 (Mb.Blh) were integrated into the rDNA sites of the β-carotene overproducer. The resulting strain produced more retinol than retinal, suggesting strong endogenous promiscuous RDH activity in Y. lipolytica. The introduction of Mb.Blh led to a considerable reduction in β-carotene level, but less than 5% of the consumed β-c
Who reads Efficient production of retinol in Yarrowia lipolytica by increasing stability using antioxidant and detergent extraction?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hyemin Park; Dongpil Lee; Jae-Eung Kim; Seonmi Park; Joo Hyun Park; Cheol Woong Ha; Minji Baek; Seok-Hwan Yoon; Kwang Hyun Park; Peter Lee; Ji-Sook Hahn
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)