Opening book details…
Can I read Utility of electrophoretically derived protein mass estimates as additional constraints in proteome analysis of human serum based on MS/MS analysis on EtoBox?
Utility of electrophoretically derived protein mass estimates as additional constraints in proteome analysis of human serum based on MS/MS analysis by Jin Young Kim; Jeong Hwa Lee; Gun Wook Park; Kun Cho; Kyung-Hoon Kwon; Young Mok Park; Sang Yun Cho; Young-Ki Paik; Jong Shin Yoo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Utility of electrophoretically derived protein mass estimates as additional constraints in proteome analysis of human serum based on MS/MS analysis about?
## Abstract The proteome of a HUPO human serum reference sample was analyzed using multidimensional separation techniques at both the protein and the peptide levels. To eliminate false‐positive identifications from the search results, we employed a data filtering method using molecular weight (MW) correlations derived from denaturing 1‐DE. First, the six most abundant serum proteins were removed from the sample using immunoaffinity chromatography. 1‐DE was then used to fractionate the remaining serum proteins according to the MW. Gel bands were isolated and in‐gel digested with trypsin, and the resulting peptides were analyzed by 2‐D LC/ESI‐MS/MS. A SEQUEST search using the MS/MS results identified 494 proteins. Of these, 202 were excluded formally using protein data filtering as they were single‐assignment proteins and their theoretical and electrophoretically‐derived MWs did not correlate at high confidence. To evaluate this method, the results were compared with those of 1‐D LC/MALDI‐TOF/TOF and HUPO Plasma Proteome Project analyses. Our data filtering approach proved valuable in analysis of complex, large‐scale proteomes such as human serum.
Who reads Utility of electrophoretically derived protein mass estimates as additional constraints in proteome analysis of human serum based on MS/MS analysis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jin Young Kim; Jeong Hwa Lee; Gun Wook Park; Kun Cho; Kyung-Hoon Kwon; Young Mok Park; Sang Yun Cho; Young-Ki Paik; Jong Shin Yoo
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)