Can I read Simplifying lymphocyte culture and the deoxyuridine suppression test by using whole blood (0.1 mL) instead of separated lymphocytes. on EtoBox?
Simplifying lymphocyte culture and the deoxyuridine suppression test by using whole blood (0.1 mL) instead of separated lymphocytes. by Das, K C; Manusselis, C; Herbert, V is a Medicine article available to read on EtoBox.
What is Simplifying lymphocyte culture and the deoxyuridine suppression test by using whole blood (0.1 mL) instead of separated lymphocytes. about?
We describethe use ofsmallvolumes (0.1mL) ofwhole blood instead of separated lymphocytes, in a microscale technique of lymphocyte culture. We incubated at 37 #{176}C 0.1 mL of heparinized blood in 0.9 mL of 60 mmol/L tris(hydroxymethyl)methylamine-buffered Hanks-Eagle medium (pH 7.4)unsupplementedwithfetal calfserum. ## Incorporation of [3H]thymidine or [125l]deoxyuridine into the DNA of 2 X i0 to 3.5 X iO lymphocytes was greatest with 10-20 ug of phytohemagglutinin-P per culture tube. ## Radionucleoside incorporation peaked in normal lymphocytes on day 4 (i.e., a day later than in culture of separated lymphocytes) and on day 8 in lymphocytes from cases of chronic lymphocytic leukemia. Lymphocyte transformation in whole-blood culture was qualitatively similar to that in cultures of separated lymphocytes from the same person. Five to 10 mol of nonradioactive deoxyuridine per culture was needed to suppress to about 10% of control the incorporation of subsequently added [3H]thymidine or [125l]deoxyuridine into DNA. Shortening the incubation to 1 h did not significantly affect qualitative results of the deoxyuridine suppression test. Whole-blood culture issimple, requires less blood, e
Who reads Simplifying lymphocyte culture and the deoxyuridine suppression test by using whole blood (0.1 mL) instead of separated lymphocytes.?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Das, K C; Manusselis, C; Herbert, V
- Publisher
- American Association for Clinical Chemistry; American Association for Clinical Chemistry, Inc.; Oxford University Press (OUP); American Association for Clinical Chemistry (AACC); Test accounts (ISSN 0009-9147)
- Published
- 1980
- Language
- EN
- Field
- Medicine (Health Sciences)