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‘Phytoantibodies’: a general vector for the expression of immunoglobulin domains in transgenic plants by Eugenio Benvenuto; Ricardo J. Ordàs; Raffaela Tavazza; Giorgio Ancora; Silvia Biocca; Antonino Cattaneo; Patrizia Galeffi is a Agricultural and Biological Sciences article available to read on EtoBox.

What is ‘Phytoantibodies’: a general vector for the expression of immunoglobulin domains in transgenic plants about?

Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immunohistochemical analysis. Immunoblotting showed that the polypeptide was stably expressed and accounted for up to 1 ~o of the soluble protein fraction. It is therefore proposed that single immunoglobulin domains of suitable specificity expressed in plants may constitute an effective system to inhibit the activity of molecules involved in plant pathology or plant development.

Who reads ‘Phytoantibodies’: a general vector for the expression of immunoglobulin domains in transgenic plants?

Author

Eugenio Benvenuto; Ricardo J. Ordàs; Raffaela Tavazza; Giorgio Ancora; Silvia Biocca; Antonino Cattaneo; Patrizia Galeffi

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1991

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)