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Quantitation of Urinary 7-Methyladenine by Gas Chromatography-Mass Spectrometry Using Isotopically Labeled Internal Standards by H.G. Mandel; J.J. Kusmierz; B.F. Dickens; L.W. Anderson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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We have developed a procedure for isolating and quantifying 7 -methyladenine from rat urine following the administration to the rat of methylating agents, such as dimethylnitrosamine. Urinary 7-methyladenine and its trideutero isomer, added as an internal standard, were precipitated with silver nitrate, the precipitate was extracted with \(\mathrm{HCl}\), and the extract was further purified by \(\mathrm{C}_{18}\)-Sep-Pak chromatography. The recovered 7 -methyladenine was then derivatized with pentafluorobenzyl bromide at alkaline \(\mathrm{pH}\) for analysis by gas chromatography-mass spectrometry, indicating a bis(pentafluorobenzyl) conjugate, \(m / z 509\). The mass spectrum of this derivative shows a major fragmentation ion at \(m / z 328\) (and 331 for the trideutero derivative) resulting from the loss of one pentafluorobenzyl group. Levels of urinary 7 -methyladenine above \(150 \mathrm{pg}\) could be detected from the ratio of the gas chromatography peak areas for these ions, using selective-ion monitoring. The method was selective for the 7 -methyl isomer. The procedures developed for the syntheses of deuterated and tritiated 7 -methyladenine, which were required for these

Who reads Quantitation of Urinary 7-Methyladenine by Gas Chromatography-Mass Spectrometry Using Isotopically Labeled Internal Standards?

Author

H.G. Mandel; J.J. Kusmierz; B.F. Dickens; L.W. Anderson

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)