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Protective Effect of Human Amniotic Membrane Extract Against Hydrogen Peroxide‐induced Oxidative Damage in Human Dermal Fibroblasts by Negin Talachi; Elaheh Afzal; Masoumeh Nouri; Saeid Abroun; Morteza Zarrabi; Hoda Jahandar is a Medicine article available to read on EtoBox.

What is Protective Effect of Human Amniotic Membrane Extract Against Hydrogen Peroxide‐induced Oxidative Damage in Human Dermal Fibroblasts about?

## Objective: One of the main approaches to preventing skin ageing is to protect fibroblast cells from oxidative stress. the promoting effect of the human amniotic membrane extract (hame) on re-epithelization, proliferation and migration of cells in wound healing has been already well studied. this experimental study aimed to investigate the antioxidant activity of hame against hydrogen peroxide (h o )-induced dermal fibroblast damage. ## Methods: Here, to establish the ageing model, human foreskin fibroblasts (hffs) were exposed to 200 μm h o for 2 h. hffs were treated with 0.1 mg/ml ame for 24 or 48 h before or/and after h o exposure. a total of 48 h following the h o treatment, we measured cell proliferation, viability, senescence-associated β-galactosidase (sa-β-gal), antioxidants and preinflammatory cytokine (il-6) levels, as well as the expression of senescence-associated genes (p53 and p21). ## Results: The obtained results indicated that under oxidative stress, ame significantly increased cellular viability and not only promoted the cell proliferation rate but also attenuated apoptotic induction condition (p < 0.001). ame also significantly reversed the sa-β-gal levels indu

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Author

Negin Talachi; Elaheh Afzal; Masoumeh Nouri; Saeid Abroun; Morteza Zarrabi; Hoda Jahandar

Published

2022

Language

EN

Field

Medicine (Health Sciences)