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Tryptic digest analysis by comprehensive reversed phase×two reversed phase liquid chromatography (RP‐LC×2RP‐LC) at different pH's by Isabelle François; Deirdre Cabooter; Koen Sandra; Frédéric Lynen; Gert Desmet; Pat Sandra is a Chemistry article available to read on EtoBox.
What is Tryptic digest analysis by comprehensive reversed phase×two reversed phase liquid chromatography (RP‐LC×2RP‐LC) at different pH's about?
## Abstract As an alternative to the classical approach of combining strong cation exchange SCX‐LC and RP‐LC for the separation of complex proteomic samples, this essay describes the online comprehensive RP‐LC×RP‐LC separation of BSA and human blood serum. High orthogonality and peak capacity are achieved through the application of a significantly different pH in the two dimensions. The coupling of fused‐core columns in series ensures high efficiency in the first dimension, while a previously designed interface with parallel second dimension columns further enhances the separation capability of the comprehensive system.
Who reads Tryptic digest analysis by comprehensive reversed phase×two reversed phase liquid chromatography (RP‐LC×2RP‐LC) at different pH's?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Isabelle François; Deirdre Cabooter; Koen Sandra; Frédéric Lynen; Gert Desmet; Pat Sandra
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1615-9306)
- Published
- 2009
- Language
- EN
- Field
- Chemistry (Physical Sciences)