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Reactivating chaperones for coenzyme B12-dependent diol and glycerol dehydratases and ethanolamine ammonia-lyase by Tetsuo Toraya; Takamasa Tobimatsu; Naoki Shibata; Koichi Mori is a book available to read on EtoBox.

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Role of reactivating chaperones in coenzyme recycling: Activity-maintaining system 266 8.1 Coenzyme recycling 266 8.2 Activity-maintaining system and coenzyme recycling 267 9. Protocol 268 9.1 Assay of in situ reactivation by measuring time-course of reaction with permeabilized cells 268 9.2 Assay of in situ (re)activation of inactivated holoenzymes (A) and inactive enzyme•cobalamin complexes (B) 269 9.3 In situ functional analysis of reactivating factor genes by co-expression experiments 270 9.4 Assay of in vitro reactivation of suicidally-inactivated holoenzymes (A) and holoenzymes inactivated in the absence of substrates (B) 270 9.5 Assay of in vitro activation of inactive enzyme•cobalamin complexes 271 9.6 Purification of recombinant wild-type DD-R and EAL-R 272 9.7 Purification of recombinant wild-type GD-R 273 9.8 Spectral confirmation of the exchange of enzyme-bound CN-Cbl for AdePeCbl 274 9.9 Verification of cobalamin exchange mechanism using radiolabeled CN-Cbl 274 9.10 Intermediate formation of apoenzymes from inactivated holoenzymes (A) and model complexes (B) 275 9.11 Evaluation of complex formation between apoenzymes and reactivating factors 276 9.12 Crystallization 27

Author

Tetsuo Toraya; Takamasa Tobimatsu; Naoki Shibata; Koichi Mori

Publisher

Academic Press, an imprint of Elsevier

Published

2022

Language

EN

ISBN

9780323915922

Subjects

Science, Chemistry, Biology