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Can I read Novel High-resolution Targeted Sequencing of the Cervicovaginal Microbiome on EtoBox?
Novel High-resolution Targeted Sequencing of the Cervicovaginal Microbiome by Karolina M. Andralojc; Mariano A. Molina; Mengjie Qiu; Bram Spruijtenburg; Menno Rasing; Bernard Pater; Martijn A. Huynen; Bas E. Dutilh; Thomas H. A. Ederveen; Duaa Elmelik; Albert G. Siebers; Diede Loopik; Ruud L. M. Bekkers; William P. J. Leenders; Willem J. G. Melchers is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Novel High-resolution Targeted Sequencing of the Cervicovaginal Microbiome about?
Abstract Background The cervicovaginal microbiome (CVM) plays a significant role in women’s cervical health and disease. Microbial alterations at the species level and characteristic community state types (CST) have been associated with acquisition and persistence of high-risk human papillomavirus (hrHPV) infections that may result in progression of cervical lesions to malignancy. Current sequencing methods, especially most commonly used multiplex 16S rRNA gene sequencing, struggle to fully clarify these changes because they generally fail to provide sufficient taxonomic resolution to adequately perform species-level associative studies. To improve CVM species designation, we designed a novel sequencing tool targeting microbes at the species taxonomic rank and examined its potential for profiling the CVM. Results We introduce an accessible and practical circular probe-based RNA sequencing (CiRNAseq) technology with the potential to profile and quantify the CVM. In vitro and in silico validations demonstrate that CiRNAseq can distinctively detect species in a mock mixed microbial environment, with the output data reflecting its ability to estimate microbes’ abundance. Moreover, comp
Who reads Novel High-resolution Targeted Sequencing of the Cervicovaginal Microbiome?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Karolina M. Andralojc; Mariano A. Molina; Mengjie Qiu; Bram Spruijtenburg; Menno Rasing; Bernard Pater; Martijn A. Huynen; Bas E. Dutilh; Thomas H. A. Ederveen; Duaa Elmelik; Albert G. Siebers; Diede Loopik; Ruud L. M. Bekkers; William P. J. Leenders; Willem J. G. Melchers
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)