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Auxin Exposure Disrupts Feeding Behavior and Fatty Acid Metabolism in Adult Drosophila by Sophie A. Fleck; Rebecca L. Knuteson; Emily D. Dewitt; Robert C. Eisman; Travis Nemkov; Angelo D’Alessandro; Jason M. Tennessen; Lesley N. Weaver is a scholarly article available to read on EtoBox.

What is Auxin Exposure Disrupts Feeding Behavior and Fatty Acid Metabolism in Adult Drosophila about?

The ease of genetic manipulation in __Drosophila melanogaster__ using the __Gal4/UAS__ system has been beneficial in addressing key biological questions. Current modifications of this methodology to temporally induce transgene expression require temperature changes or exposure to exogenous compounds, both of which have been shown to have detrimental effects on physiological processes. The recently described auxin-inducible gene expression system (AGES) utilizes the plant hormone auxin to induce transgene expression and is proposed to be the least toxic compound for genetic manipulation, with no obvious effects on __Drosophila__ development and survival in one wild-type strain. Here we show that auxin delays larval development in another widely-used fly strain, and that short- and long-term auxin exposure in adult __Drosophila__ induces observable changes in physiology and feeding behavior. We further reveal a dosage response to adult survival upon auxin exposure, and that the recommended auxin concentration for AGES alters feeding activity. Furthermore, auxin fed male and female flies exhibit a significant decrease in triglyceride levels and display altered transcription of fatty a

Author

Sophie A. Fleck; Rebecca L. Knuteson; Emily D. Dewitt; Robert C. Eisman; Travis Nemkov; Angelo D’Alessandro; Jason M. Tennessen; Lesley N. Weaver

Publisher

Cold Spring Harbor Laboratory

Published

2023

Language

EN