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Lentivirus Gene Engineering Protocols (Methods in Molecular Biology) by Maurizio Federico (auth.), Maurizio Federico (eds.) is a nonfiction available to read on EtoBox.

What is Lentivirus Gene Engineering Protocols (Methods in Molecular Biology) about?

Lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such

Who reads Lentivirus Gene Engineering Protocols (Methods in Molecular Biology)?

Author

Maurizio Federico (auth.), Maurizio Federico (eds.)

Publisher

Humana Press

Published

2003

Language

EN

ISBN

9781588290915

Category

nonfiction

Subjects

Science, Medical, Engineering