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Nickel-induced proteins in human HaCaT keratinocytes: annexin II and phosphoglycerate kinase by Fernando Acevedo; Miguel Ángel Serra; Monica Ermolli; Libero Clerici; Olof Vesterberg is a Environmental Science article available to read on EtoBox.

What is Nickel-induced proteins in human HaCaT keratinocytes: annexin II and phosphoglycerate kinase about?

It has been established in previous in vitro experiments with human HaCaT keratinocytes that nickel becomes cytotoxic at concentrations higher than 100 mM and that it is accumulated mainly in the cytosolic fraction . The aim of this work was to search possible biomarkers of metal insult, i.e. nickel-binding proteins or proteins differentially expressed in the cytosolic fraction of nickel-exposed cells (up to 1 mM nickel) as compared to controls. Cytosolic proteins were studied by isoelectric focusing (IEF) and two-dimensional gel electrophoresis (2-DE). Separation by IEF revealed nickel-induced changes in the abundance of cytosolic proteins as visualised with nickel-nitrilo-triacetic-alkaline phosphatase (Ni-NTA-AP) in blots. The cytosolic fraction of cells incubated with nickel, at concentrations over 100 mM, showed nickel binding components which were absent or present in significantly lower amounts in control cells. These proteins had isoelectric points (pIs) 6.9, 7.7 and 8.5. After 2-DE silver-and protein staining significantly increased abundance of four proteins was observed. Their pI values corresponded to those of the nickel binding ones seen after IEF. A protein with pI 6.

Who reads Nickel-induced proteins in human HaCaT keratinocytes: annexin II and phosphoglycerate kinase?

Author

Fernando Acevedo; Miguel Ángel Serra; Monica Ermolli; Libero Clerici; Olof Vesterberg

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0300-483X)

Published

2001

Language

EN

Field

Environmental Science (Life Sciences)