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Magnetic Nanoparticles for Imaging Dendritic Cells by Saho Kobukai; Richard Baheza; Jared G. Cobb; Jack Virostko; Jingping Xie; Amelie Gillman; Dmitry Koktysh; Denny Kerns; Mark Does; John C. Gore; Wellington Pham is a Medicine article available to read on EtoBox.

What is Magnetic Nanoparticles for Imaging Dendritic Cells about?

## Abstract We report the development of superparamagnetic iron oxide (SPIOs) nanoparticles and investigate the migration of SPIO‐labeled dendritic cells (DCs) in a syngeneic mouse model using magnetic resonance (MR) imaging. The size of the dextran‐coated SPIO is roughly 30 nm, and the DCs are capable of independent uptake of these particles, although not at levels comparable to particle uptake in the presence of a transfecting reagent. On average, with the assistance of polylysine, the particles were efficiently delivered inside DCs within one hour of incubation. The SPIO particles occupy approximately 0.35% of cell surface and are equivalent to 34.6 pg of iron per cell. __In vivo__ imaging demonstrated that the labeled cells migrated from the injection site in the footpad to the corresponding popliteal lymph node. The homing of labeled cells in the lymph nodes resulted in a signal drop of up to 79%. Furthermore, labeling DCs with SPIO particles did not compromise cell function, we demonstrated that SPIO‐enhanced MR imaging can be used to track the migration of DCs effectively __in vivo__. Magn Reson Med 63:1383–1390, 2010. © 2010 Wiley‐Liss, Inc.

Who reads Magnetic Nanoparticles for Imaging Dendritic Cells?

Author

Saho Kobukai; Richard Baheza; Jared G. Cobb; Jack Virostko; Jingping Xie; Amelie Gillman; Dmitry Koktysh; Denny Kerns; Mark Does; John C. Gore; Wellington Pham

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0740-3194)

Published

2010

Language

EN

Field

Medicine (Health Sciences)