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Preparation and Properties of an Alpha‐1‐protease Inhibitor Concentrate with High Specific Activity by E. Mattes; H. P. Matthiessen; P. L. Turecek; H. P. Schwarz is a Medicine article available to read on EtoBox.

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## Background and Objectives Because the current demand for alpha‐1‐protease inhibitor (A1PI) exceeds the available supply, we aimed to develop a process for purification of A1PI from plasma which would achieve the highest possible degree of purity, specific activity and yield. ## Materials and Methods A1PI was purified from Cohn fraction IV‐1,4 using ethanol precipitation and Q‐Sepharose chromatography. Ceramic hydroxyapatite chromatography was used as a final purification step. Two independent virus‐inactivation procedures (chemical and vapour heating) were applied. ## Results The resulting A1PI had an unprecedented high specific activity. In addition, the process led to the discovery of a new isoform of A1PI in isoelectric focusing gels. ## Conclusion The high specific activity of the A1PI preparation achieved with this process should allow a reduction of the A1PI total protein load necessary to achieve clinically relevant effects.

Who reads Preparation and Properties of an Alpha‐1‐protease Inhibitor Concentrate with High Specific Activity?

Author

E. Mattes; H. P. Matthiessen; P. L. Turecek; H. P. Schwarz

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0042-9007)

Published

2001

Language

EN

Field

Medicine (Health Sciences)