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What is Bacterial Transformation Lab Overview about?

This lab involves transforming E. coli cells with plasmids containing genes for antibiotic resistance. Students will insert a plasmid containing a gene for ampicillin resistance into competent E. coli cells. Transformed cells will be selected by plating on nutrient medium containing ampicillin. Only cells containing the plasmid will grow as they will be resistant to ampicillin. Students will calculate the transformation efficiency which expresses the number of antibiotic resistant colonies per microgram of

Author

Chris Price

Language

EN