Opening book details…
Can I read Qualitative and quantitative determination of seven triterpene acids in Eriobotrya japonica Lindl. by high‐performance liquid chromatography with photodiode array detection and mass spectrometry on EtoBox?
Qualitative and quantitative determination of seven triterpene acids in Eriobotrya japonica Lindl. by high‐performance liquid chromatography with photodiode array detection and mass spectrometry by Er‐Na Li; Jian‐Guang Luo; Ling‐Yi Kong is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Qualitative and quantitative determination of seven triterpene acids in Eriobotrya japonica Lindl. by high‐performance liquid chromatography with photodiode array detection and mass spectrometry about?
## Abstract ## Introduction The leaves of __Eriobotrya japonica__ are used for the treatment of diabetes mellitus, chronic bronchitis, coughs and skin diseases. No method is currently available, however, by which to assess the quality of the crude herb on the basis of the quantitative profile of the main bioactive triterpene acids present. ## Objective To develop a simple and accurate HPLC‐UV (photodiode array detection) method for the simultaneous quantification of seven triterpene acids in the leaves of __E. japonica__. ## Method Separations were performed on an Ultimate XB‐C~18~ column by gradient elution using methanol:formic acid. Analytes were identified by HPLC coupled with electrospray ionisation mass spectrometry experiments. The quantitative HPLC‐UV method was validated for linearity, precision, accuracy, and limits of detection and quantification. ## Results Calibration curves presented good linear regression (__r__ > 0.9992) within test ranges. The precision and accuracy of the method were acceptable with overall intra‐day and inter‐day variations of 1.35–3.30 and 1.98–4.43%, respectively, and overall recoveries of 95.60–102.67% for the seven compounds analysed. The met
Who reads Qualitative and quantitative determination of seven triterpene acids in Eriobotrya japonica Lindl. by high‐performance liquid chromatography with photodiode array detection and mass spectrometry?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Er‐Na Li; Jian‐Guang Luo; Ling‐Yi Kong
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0958-0344)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)