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Analyses of Damage-associated Molecular Patterns, Particularly Biglycan, in Cisplatin-induced Rat Progressive Renal Fibrosis by Minto Nakagawa; Takeshi Izawa; Mitsuru Kuwamura; Jyoji Yamate is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Damage-associated molecular patterns (DAMPs) and their receptors (TLR-2 and -4) may play important roles in renal fibrosis, of which the pathogenesis is complicated. We used rat renal lesions induced by a single intraperitoneal injection of cisplatin at 6 mg/kg body weight; consisting of tissue damage of renal tubules on days 1 and 3, further damage and regeneration with inflammation mainly on days 5 and 7, and interstitial fibrosis on days 9, 12, 15, and 20. Microarray analyses on days 5 (the commencement of inflammation) and 9 (the commencement of interstitial fibrosis) showed that DAMPs increased by more than two-fold relative to control included common extra-cellular matrix (ECM) components such as laminin (Lamc2) and fibronectin, and heat shock protein family, as well as fibrinogen, although it was limited analysis; Lamc2, an element of basement membrane, may be regarded as an indicator for damaged renal tubules. In the real-time RT-PCR analyses, TLR-2 significantly increased transiently on day 1, whereas TLR-4 significantly increased on days 9 and 15, almost in agreement with the increased biglycan (a small leucine-rich proteoglycan as ubiquitous ECM component). As M1/M2 macr

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Author

Minto Nakagawa; Takeshi Izawa; Mitsuru Kuwamura; Jyoji Yamate

Publisher

Japanese Society of Toxicologic Pathology

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)