Can I read Precision measurement of ternary diffusion coefficients and implications for protein crystal growth: lysozyme chloride in aqueous ammonium chloride at 25°C on EtoBox?

Precision measurement of ternary diffusion coefficients and implications for protein crystal growth: lysozyme chloride in aqueous ammonium chloride at 25°C by Luigi Paduano; Onofrio Annunziata; Arne J Pearlstein; Donald G Miller; John G Albright is a Engineering article available to read on EtoBox.

What is Precision measurement of ternary diffusion coefficients and implications for protein crystal growth: lysozyme chloride in aqueous ammonium chloride at 25°C about?

Isothermal ternary diffusion coefficients for the system lysozyme chloride (1)+NH 4 Cl (2)+H 2 O at 258C and pH 4.5 have been measured interferometrically for five mean NH 4 Cl concentrations, C 2 1⁄4 0:25, 0.5, 0.9, 1.2 and 1.5 M, with C 1 1⁄4 0:6 mM. The main-term diffusion coefficient ðD 11 Þ v varies slowly with C 2 . The main-term ðD 22 Þ v increases with increasing NH 4 Cl concentration, as does the binary D v in aqueous NH 4 Cl, but the ðD 22 Þ v values are lower in the ternary system. The cross-term ðD 21 Þ v , which relates the coupled flow of NH 4 Cl to the protein concentration gradient, increases sharply with increasing salt concentration, and is 19 times larger than ðD 22 Þ v at the highest concentration. The values of ðD 12 Þ v are smaller than the corresponding values previously obtained for the lysozyme chloride+NaCl+H 2 O system over the whole range of salt concentration studied. Using equations based on the Onsager Reciprocal Relations, we have calculated the derivative of the chemical potential of lysozyme chloride with respect to the NH 4 Cl concentration, and have estimated the protein cation charge. Integration with respect to the NH 4 Cl concentration gives t

Who reads Precision measurement of ternary diffusion coefficients and implications for protein crystal growth: lysozyme chloride in aqueous ammonium chloride at 25°C?

Author

Luigi Paduano; Onofrio Annunziata; Arne J Pearlstein; Donald G Miller; John G Albright

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0022-0248)

Published

2001

Language

EN

Field

Engineering (Physical Sciences)