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Influence of Distal Glycan Mimics on Direct Electron Transfer Performance for Bilirubin Oxidase Bioelectrocatalysts by Sayaka Nishida; Hinata Sumi; Haruna Noji; Akira Itoh; Kunishige Kataoka; Satoshi Yamashita; Kenji Kano; Keisei Sowa; Yuki Kitazumi; Osamu Shirai is a Engineering article available to read on EtoBox.
What is Influence of Distal Glycan Mimics on Direct Electron Transfer Performance for Bilirubin Oxidase Bioelectrocatalysts about?
Bilirubin oxidase (BOD) is a bioelectrocatalyst that reduces dioxygen (O2) to water and is capable of direct electron transfer (DET)-type bioelectrocatalysis via its electrode-active site (T1 Cu). BOD from Myrothecium verrucaria (mBOD) has been widely studied and has strong DET activity. mBOD contains two N-linked glycans (N-glycans) with N472 and N482 binding sites distal to T1 Cu. We previously reported that different N-glycan compositions affect the enzymatic orientation on the electrode by using recombinant BOD expressed in Pichia pastoris and the deglycosylation method. However, the individual function of the two N-glycans and the effects of N-glycan composition (size, structure, and non-reducing termini) on DET-type reactions are still unclear. In this study, we utilize maleimide-functionalized polyethylene glycol (MAL-PEG) as an N-glycan mimic to evaluate the aforementioned effects. Site-specific enzyme-PEG crosslinking was carried out by specific binding of maleimide to Cys residues. Recombinant BOD expressed in Escherichia coli (eBOD), which does not have a glycosylation system, was used as a benchmark to evaluate the effect. Site-directed mutagenesis of Asn residue (N472
Who reads Influence of Distal Glycan Mimics on Direct Electron Transfer Performance for Bilirubin Oxidase Bioelectrocatalysts?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Sayaka Nishida; Hinata Sumi; Haruna Noji; Akira Itoh; Kunishige Kataoka; Satoshi Yamashita; Kenji Kano; Keisei Sowa; Yuki Kitazumi; Osamu Shirai
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Engineering (Physical Sciences)