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The Erwinia chrysanthemi phoP-phoQ operon plays an important role in growth at low pH, virulence and bacterial survival in plant tissue by Arancha Llama-Palacios; Emilia López-Solanilla; César Poza-Carrión; Francisco García-Olmedo; Pablo Rodríguez-Palenzuela is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Summary We have studied the role of acidic pH as a barrier for the colonization of the plant apoplast by __Erwinia chrysanthemi__. A minitransposon containing a promoterless reporter gene, __gus__, was used for random mutagenesis of the bacterial genome. An acid‐sensitive mutant, named BT119, was isolated and had the following differential features with respect to the wild‐type strain: (i) inability to grow at pH ≤ 5.5; (ii) decreased survival at acid pH and in plant tissues; (iii) increased susceptibility to antimicrobial peptides; (iv) decreased virulence in chicory leaves and pear fruits; (v) reduced polygalacturonase production; and (vi) reduced ability to alkalinize chicory tissues after infection. The sequence of the interrupted gene was highly similar to the __phoQ__ gene, which is involved in environmental sensing in several bacteria, such as __Yersinia pseudotuberculosis__, __Erwinia carotovora__, __Salmonella typhimurium__ and __Escherichia coli__ and thus, this designation was used for the __E. chrysanthemi__ system. This gene was induced at low Mg^2+^ concentrations and __in planta__. These results suggest that __E. chrysanthemi__ PhoP‐PhoQ system plays an important
Who reads The Erwinia chrysanthemi phoP-phoQ operon plays an important role in growth at low pH, virulence and bacterial survival in plant tissue?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Arancha Llama-Palacios; Emilia López-Solanilla; César Poza-Carrión; Francisco García-Olmedo; Pablo Rodríguez-Palenzuela
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)
- Published
- 2003
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)