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Can I read Isolation and characterization of mitochondrial DNA of the oomycetous fungus Phytophthora infestans on EtoBox?
Isolation and characterization of mitochondrial DNA of the oomycetous fungus Phytophthora infestans by L. J. Klimczak; H. H. Prell is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Isolation and characterization of mitochondrial DNA of the oomycetous fungus Phytophthora infestans about?
Mitochondrial DNA (mtDNA) of Phytophthora infestans has been isolated and preliminarily characterized. It has a low GC content of about 22.4% which is distinctly different from that of nuclear DNA (51%). This property has been used to separate both DNA species in the presence of 4',6-diamidine-2-phenylindole (DAPI) in CsC1 density gradients. The use of cetyltriammonium bromide (CTAB) for extraction of DNA significantly reduced its degradation. The base distribution of the mtDNA shows a limited intramolecular heterogeneity. The molecule contains 36.2 -+ 0.3 kb as revealed by endonuclease digestion and seems to be circular as shown by restriction mapping. No differences were found in restriction patterns between mtDNAs from various pathotypes.
Who reads Isolation and characterization of mitochondrial DNA of the oomycetous fungus Phytophthora infestans?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- L. J. Klimczak; H. H. Prell
- Publisher
- Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0172-8083)
- Published
- 1984
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)