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**Subtype‐specific interactions of type C staphylococcal enterotoxins with the T‐cell receptor** by James R. Deringer; R. James Ely; Cynthia V. Stauffacher; Gregory A. Bohach is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is **Subtype‐specific interactions of type C staphylococcal enterotoxins with the T‐cell receptor** about?

The goal of this study was to investigate the molecular interaction between superantigens and the T‐cell receptor (TCR). Using a quantitative polymerase chain reaction (PCR) to assess T‐cell proliferation profiles, we found that SEB, SEC1, SEC2 and SEC3 expanded human T cells bearing Vβ3, Vβ12, Vβ13.2, Vβ14, Vβ15, Vβ17 and Vβ20. SEC2 and SEC3 have the additional ability to expand T cells bearing Vβ13.1, and their expansion of Vβ3 was markedly reduced compared to SEB and SEC1. Based on the activity of SEC1 mutants containing single amino acid substitutions, we concluded that the differential abilities of these native toxins to stimulate Vβ3 and Vβ13.1 was determined by the residue in position 26, located in the base of the SEC α3 cavity. The SEC1 mutant, in which Val in position 26 was substituted with the analogous SEC2/SEC3 residue (Tyr), generated a Vβ expansion profile that was indistinguishable from those generated by SEC2 and SEC3. Using these findings, the co‐ordinates of a recently reported murine TCR β‐chain crystal structure, and other documented information, we propose a compatible molecular model for the interaction of SEC3 with the T‐cell receptor. In this model complex

Who reads **Subtype‐specific interactions of type C staphylococcal enterotoxins with the T‐cell receptor**?

Author

James R. Deringer; R. James Ely; Cynthia V. Stauffacher; Gregory A. Bohach

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)