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Transient Expression and Heat-stress-inducedco-aggregation of Endogenous and Heterologous Small Heat-stress Proteins in Tobacco Protoplasts by Marc Kirschner; Sybille Winkelhaus; Jörg M. Thierfelder; Lutz Nover is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Transient Expression and Heat-stress-inducedco-aggregation of Endogenous and Heterologous Small Heat-stress Proteins in Tobacco Protoplasts about?

Heat-stress granules (HSG) are highly ordered, cytoplasmic chaperone complexes found in all heat-stressed plant cells. We have developed an experimental system involving expression of cytosolic class I and class II small heat-stress proteins (Hsps) of pea, Arabidopsis and tomato in tobacco protoplasts to study the structural prerequisites for the assembly of HSG or HSG-like complexes. Class I and class II small Hsps formed class-specific dodecamers of 210-280 kDa, which, upon heat stress, were incorporated into HSG complexes. Interestingly, class II dodecamers alone could form HSG-like complexes (auto-aggregation), whereas class I dodecamers could do so only in the presence of class II proteins (recruitment). By analysing C-terminal deletion forms of Hsp17 class II, we obtained evidence that the intact C-terminus is critical for the oligomerization state, for the heat-stress-induced auto-aggregation and for recruitment of class I proteins. The class-specific formation of dimers as a prerequisite for oligomerization was analysed by the yeast two-hybrid system. In the presence of the endogenous (tobacco) set of heat-stress-induced proteins, all heterologous class I and class II prote

Who reads Transient Expression and Heat-stress-inducedco-aggregation of Endogenous and Heterologous Small Heat-stress Proteins in Tobacco Protoplasts?

Author

Marc Kirschner; Sybille Winkelhaus; Jörg M. Thierfelder; Lutz Nover

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

2000

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)