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Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine by Cecilia Hegardt; Oskar T. Johannsson; Stina M. Oredsson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine about?

The spermine analogue __N__^1^,__N__^11^‐diethylnorspermine (DENSPM) efficiently depletes the cellular pools of putrescine, spermidine and spermine by down‐regulating the activity of the polyamine biosynthetic enzymes and␣up‐regulating the activity of the catabolic enzyme spermidine/ spermine __N__^1^‐acetyltransferase (SSAT). In the breast cancer cell line L56Br‐C1, treatment with 10 μm DENSPM induced SSAT activity 60 and 240‐fold at 24 and 48 h after seeding, respectively, which resulted in polyamine depletion. Cell proliferation appeared to be totally inhibited and within 48 h of treatment, there was an extensive apoptotic response. Fifty percent of the cells were found in the sub‐G~1~ region, as determined by flow cytometry, and the presence of apoptotic nuclei was morphologically assessed by fluorescence microscopy. Caspase‐3 and caspase‐9 activities were significantly elevated 24 h after seeding. At 48 h after seeding, caspase‐3 and caspase‐9 activities were further elevated and at this time point a significant activation of caspase‐8 was also found. The DENSPM‐induced cell death was dependent on the activation of the caspases as it was inhibited by the general caspase inhibi

Who reads Rapid caspase-dependent cell death in cultured human breast cancer cells induced by the polyamine analogue N1,N11-diethylnorspermine?

Author

Cecilia Hegardt; Oskar T. Johannsson; Stina M. Oredsson

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)