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Effects of Polyamines on Apoptosis Induced by Simulated Ischemia/reperfusion Injury in Cultured Neonatal Rat Cardiomyocytes by Liping Han; Changqing Xu; Chunming Jiang; Hongzhu Li; Weihua Zhang; Yajun Zhao; Li Zhang; Yanqiao Zhang; Weiming Zhao; Baofeng Yang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Effects of Polyamines on Apoptosis Induced by Simulated Ischemia/reperfusion Injury in Cultured Neonatal Rat Cardiomyocytes about?
## Abstract We incubated neonatal Sprague—Dawley rat cardiomyocytes in primary culture in a medium simulating ischemia (consisting of hypoxia plus serum deprivation) for 2 h, then re‐incubated them for 24 h in normal culture medium to establish a model of simulated ischemia/reperfusion (I/R) injury. Apoptotic cell death was measured by MTT assay, TUNEL staining and flow cytometry. Morphological alterations were assessed by transmission electron microscopy, the expression of caspases‐3 and −9 and Bcl‐2 and the release of cytochrome __c__ by Western blotting, and the intracellular free‐calcium concentration ([Ca^2+^]~i~) by laser confocal scanning microscopy. The results showed that pretreatment with 10 μmol/l spermine or spermidine significantly inhibited apoptosis in the I/R cells, suppressed the expression of caspases‐3 and −9 and cytochrome __c__ release, up‐regulated Bcl‐2 expression and decreased [Ca^2+^]~i~. However, pretreatment with 10 μmol/l putrescine had the opposite effects. Evidence for this “double‐edged” effect of polyamines on apoptosis in I/R‐injured cardiomyocytes is presented for the first time. It may suggest a novel pharmacological target for preventing and trea
Who reads Effects of Polyamines on Apoptosis Induced by Simulated Ischemia/reperfusion Injury in Cultured Neonatal Rat Cardiomyocytes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Liping Han; Changqing Xu; Chunming Jiang; Hongzhu Li; Weihua Zhang; Yajun Zhao; Li Zhang; Yanqiao Zhang; Weiming Zhao; Baofeng Yang
- Publisher
- Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 1065-6995)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)