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Whole Mitochondrial Genomes Assembled from Thermally Altered Forensic Bones and Teeth by M.V. Emery; K. Bolhofner; S. Ghafoor; S. Winingear; J.E. Buikstra; L.C. Fulginiti; A.C. Stone is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Whole Mitochondrial Genomes Assembled from Thermally Altered Forensic Bones and Teeth about?

The recovery and analysis of genetic material obtained from thermally altered human bones and teeth are increasingly important to forensic investigations, especially in cases where soft-tissue identification is no longer possible. Although little is known about how these fire-related processes affect DNA degradation over time, next-generation sequencing technology in combination with traditional osteobiographical applications may provide us clues to these questions. In this study, we compare whole mitochondrial genome data generated using two different DNA extraction methods from 27 thermally altered samples obtained from fire victims (Maricopa County, Arizona) . DNA extracts were converted to double-stranded DNA libraries and enriched for whole mitochondrial DNA (mtDNA) using synthetic biotinylated RNA baits, then sequenced on an Illumina MiSeq. We processed the mitochondrial data using an in-house computational pipeline (MitoPipe1.0) composed of ancient DNA and modern genomics applications, then compared the resulting information across the two extraction types and five burn categories. Our analysis shows that DNA fragmentation increases with temperature, but that the acute insul

Who reads Whole Mitochondrial Genomes Assembled from Thermally Altered Forensic Bones and Teeth?

Author

M.V. Emery; K. Bolhofner; S. Ghafoor; S. Winingear; J.E. Buikstra; L.C. Fulginiti; A.C. Stone

Publisher

Elsevier BV

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)