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Characterization of β‐defensin prepropeptide mRNA from chicken and turkey bone marrow by C. W. Brockus; M. W. Jackwood; B. G. Harmon is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Four avian β‐defensin prepropeptide cDNA sequences [gallinacins: Gal 1 (synonym CHP 1, chicken heterophil peptide 1), and Gal 2; turkey heterophil peptides: THP 1 and THP 2] were amplified from chicken or turkey bone marrow mRNA samples, respectively. Partial chicken β‐defensin cDNA sequences were obtained using degenerate primers based on chicken peptide sequences (Gal 1/CHP 1 and Gal 2). The complete cDNA sequences of the chicken β‐defensins were then determined by designing specific intrapeptidal primers, from the newly acquired sequence, and pairing one primer with a specific poly A primer tail sequence (3′ end) and the other primer with an adapter primer in a 5′ rapid amplification of cDNA ends (RACE) reaction. The two, turkey β‐defensins were amplified from turkey marrow using primers designed from chicken β‐defensin preproregions. The complete amino acid sequences for the prepropeptides were deduced for all four avian β‐defensins. Previously, only partial mature peptide sequences for the turkey β‐defensins and complete mature peptide sequences for the chicken β‐defensins were known. All sequences obtained translated accurately to complete and partial amino acid sequences rep

Who reads Characterization of β‐defensin prepropeptide mRNA from chicken and turkey bone marrow?

Author

C. W. Brockus; M. W. Jackwood; B. G. Harmon

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0268-9146)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)