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Assay of Underivatized Salicylamide in Plasma, Saliva and Urine by A.G. de Boer; J.M. Gubbens-Stibbe; F.H. de Koning; A. Bosma; D.D. Breimer is a Chemistry article available to read on EtoBox.

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Several methods have been described for the determination of salicylamide (SAM) in plasma and urine. These comprise UV spectrophotometric [L-5] , spectrofluorimetric [6] and gas-liquid chromatographic (GLC) methods 17, S] . The UV spectrophotometric methods exhibit lack of sensitivity (detection limit about 10 pg SAM per ml sample)\_ The spectrofluorimetric method is more sensitive (0.5 pg SAM per ml sample), but lacks specificity and blank values have to be subtracted [6] \_ The published GLC methods either require the formation of trimethylsilyl derivatives 173 , or lack sensitivity [S] \_ We developed a rapid and sensitive method for the determination of underivatized SAM in human plasma and saliva and rat whole blood, using gas chromatography with nitrogen selective detection and solid injection system. EXPERIMENTAL -4pparatus A Hewlett-Packard 5750 gas chromatograph was used with an alkali flame ionization detector (nitrogen detector, HP 15151A, rubidium bromide crystal). Temperatures: injection port 280" ; column 200" ; detector 380'. A silanized borosilicate column (1.20 m X 0.8 mm I.D.), filled with 2% OV-225 + 1% OV-17 on Gas-Chrom Q, with a particle size of 200-220 Hrn, w

Who reads Assay of Underivatized Salicylamide in Plasma, Saliva and Urine?

Author

A.G. de Boer; J.M. Gubbens-Stibbe; F.H. de Koning; A. Bosma; D.D. Breimer

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1979

Language

EN

Field

Chemistry (Physical Sciences)